Glyco Liver Profile publications

Papers related to the Glyco Liver Profile (all independent of Helena Biosciences):

High-Throughput Profiling of the Serum N-Glycome on Capillary Electrophoresis Microfluidics Systems: Toward Clinical Implementation of GlycoHepatoTest

Vanderschaeghe D, Szekrényes Á, Wenz C, Gassman M, Naik N, Bynum M, Yin H, Delanghe J, Guttman A, Callewaert N
Analytical Chemistry (2010) vol.82 7408-7415

Developed 3 h procedure for preparing serum N-glycans and labelling them with 8-aminopyrene-1,3,6-trisulfonic acid. This followed by successful analysis via capillary electrophoresis on three microfluidics-based platforms: MCE 202 MultiNa, 2100 Bioanalyzer and a modified prototype of the eGene system. This GlycoHepatoTest allows follow-up liver fibrosis patients from an early stage.

Noninvasive diagnosis of liver cirrhosis using DNA sequencer-based total serum protein glycomics

Callewaert N, Vlierberghe H, Hecke A, Laroy W, Delanghe J, Contreras R
Nature Medicine (2004) vol.10 chapter 4 429-434

Technologies based on DNA sequencer/fragment analysers generated a N-glycan serum protein profile in liver disease patients. This yielded a biomarker that distinguishes compensated cirrhotic from noncirrhotic chronic liver disease with high specificity and sensitivity. Especially when combined with Fibrotest biomarker.

GlycoFibroTest is a Highly Performant Liver Fibrosis Biomarker Derived from DNA Sequencer-based Serum Protein Glycomics

Vanderschaeghe D, Laroy W, Sablon E, Halfon P, Hecke A, Delanghe J, Callewaert N
MCP Papers: Molecullar and Cellular Proteomics (2009) vol.8.5 986-994

Investigation into serum N-glycome’s potential as a biomarker via 96-well plate-based serum N-glycome comics to be used in capillary electrophoresis-based DNA sequencers. A blinded study of 376 chronic hepatitis C virus patients found the ratios of two N-glycans (GlycoFibroTest) correlates with the histological fibrosis stage. Affinity chromatography was used to deplete partially determining GlycoFibroTest.

Glycome mapping on DNA sequencing equipment

Laroy W, Contreras R, Callewaert N
Nature Protocols (2006) vol.1 chapter 1 397-405

An offered detailed protocol for the analysis of protein-linked glycans on DNA sequencing equipment. The protocol utilizes mutlticapillary DNA sequencers, resolving isobaric glycan stereoisomers and facilitates two-dimensional profiling or MS analysis of particular compounds.

Glycome profiling using modern glycomics technology: technical aspects and applications

Vanderschaeghe D, Festjens N, Delanghe H, Callewaert N
Biological Chemistry (2010) vol.391 149-161

Review on glycomics research and the highlights of glycan profiling tools between 2005 and 2010. The review focuses mainly on capillary electrophoresis, liquid chromatography, mass spectrometry and lectin microarrays.

N-glycan based biomarker distinguishing non-alcoholic steatohepatitis from steatosis independently of fibrosis

Blomme B, Francque S, Trépo E, Libbrecth L, Vanderschaeghe D, Verrijken A, Pattyn P, Nieuwhenhove Y, Putte D, Geerts A, Colle I, Delanghe J, Moreno C, Gaal L, Callewaert N, Vlierberghe H
Elsevier: Digestive and Liver Disease (2012) vol.44 315-322

N-glycosylation of serum proteins used to identify NASH biomarker by assessing patterns using DNA sequencer-assiosted flrophore-assisted capillary electrophoresis and histology. The glycomarker recognized liver inflammation in obese individuals and can differentiate between steatosis and NASH.

P0175: Assessment of serum glycomics (glycocirrhotest) for risk prediction of hepatocellular carcinoma development in patients with cirrhosis

Verhelst X, Vanderschaeghe D, Castéra L, Geerts A, Goutté N, Francoz C, Durand F, Callewaert N, Vlierberghe H
Journal of Hepatology (2015) vol.62 supp. 2 365-369

132 blood samples of cirrhotic patients, majorly (70%) caused by HCV infections, were analysed. There was a significant increase in the mean baseline GlycoCirrhoTest value in the patients who developed HCC during follow up (p<0.001) compared to those that did not. Concluded as a useful biomarker for identification of high risk HCC individuals with stratified cirrhosis.

Background reading:

Alteration of protein glycosylation in liver diseases

Blomme B, Steenkiste C, Callewaert N, Vlierberghe H
Journal of Hepatology (2009) vol.50 592-603

This review explores non-invasive liver analysis via usage of protein glycolysation as pathogenic biomarkers. Individual liver diseases have specific biomarkers, hyperfucosylation, increased branching and bisecting N-acetylgucosamine modification continuously reappear in all of them. Analysis at mRNAand protein level confirms their altered status in liver pathology.

Inflammation and fibrogenesis in steatohepatitis

Fujii H, Kawada N
Journal of Gastroenterology (2012) vol.47 issue 3 215-225

Summary of biochemical and physical changes that leads to the silent symptoms of NAFLD and NASH. Information on the basic pathogenesis of Nash with a focus on inflammation and fibrosis.

Adipokines in obesity

Leal O, Mafra D
Clinica Chimica Acta (2013) vol.419 87-94

The review focuses on obesity specific-adipokine profiles and the role of some adipokines in obesity related metabolic disorders. Due to obesity’s link to increased adipose tissue, it believes adipokines play an important role in obesity related diseases such as insulin resistance, inflammation, hypertension, cardiovascular risk and metabolic disorders.

Alterations of serum protein N-glycosylation in two mouse models chronic liver disease are hepatocyte and not B cell driven

Blomme B, Steenkiste C, Grassi P, Haslam S, Dell A, Callewaert N, Vlierberghe H
American Journal of Physiology: Gastrointestinal and Liver Physiology (2011) vol.300 833-842

Induction of inflammatory cases in B cell-deficient and wild-type mice to study the cells impact in chronic liver disease. It further outlines the impact of IgG glycosylation and fibrogenesis in B cell-deficient mice.

Evaluation of the serum N-linked glycome for the diagnosis of cancer and chronic inflammation

Arnold N, Saldova R, Hamid M, Rudd M
Proteomics (2008) vol.8 no. 16 3284–3293

In this review, the glycoproteins which display glycan epitopes, the glycosyl transferases which can generate them, their potential functions and their use as biomarkers are evaluated in link with chronic inflammatory diseases and cancers.

High-throughput quantitative profiling of serum N-glycome by MALDI-TOF mass spectrometry and N-glycomic fingerprint of liver fibrosis

Kam R, Poon T, Chan H, Wong N, Hui A, Sung J
Hong Kong Medical Journal (2009) vol.15 supp. 8 42-44

Research concerning the establishment of high-throughput assay for quantitative profiling of N-glycans attached to serum glycoproteins, identification a panel of serum N-glycans as potential biomarkers for diagnosing liver cirrhosis and fibrosis and glycan peaks being able to detect these diseases with high accuracy.

Introduction of tri-antennary N-glycans in Arabidopsis thaliana plants

Nagels B, Damme E, Callewaert N, Weterings K
Plant Science (2012) vol.185 161-168

Expression of human acetylglucosaminyltransferase genes (GnT – IV and GnT – V) in fast cycling model plant Arabidopsis thaliana to synthesize tri-antennary N-glycans.

Diagnosis of Nonalcoholic Fatty Liver Disease: Invasive versus Noninvasive

Wieckowska A, Feldstein A
Seminars in Liver Disease (2008) vol.28 386-395

A review providing concise overview of the role of liver biopsy versus noninvasive diagnostic tools for the differentiation of fatty liver from non-alcoholic steatohepatitis as well as for the determination of presence and extent of fibrosis. Focuses mainly on currently available clinical practices and touches briefly on future markers under investigation.

Alteration in N-glycomics during mouse ageing: a role for FUT8

Vanhooren V, Dewaele S, Kuro-o M, Taniguchi N, Dolle L, Grunsven L, Makrantonaki E, Zouboulis C, Chen C, Libert C
Aging Cell (2011) vol.10 issue 6 1056-1066

Age-related changes in NGA2F, NA2 and NA2F in mice serum and its connection to the expression of FUT8. Lightly touches on N-glycan changes in mice serum based on calorie restriction.

Loss of α1,6-fucosyltransferase suppressed liver regeneration: implication of core fucose in the regulation of growth factor receptor-mediated cellular signalling

Wang Y, Fukada T, Isaji T, Lu J, Gu W, Lee H, Ohkubo Y,Kamada Y, Taniguchi N, Miyoshi E, Gu J
Scientific Report (2015) vol.5 article nr. 8264

Analysis of fucosylation in liver-secreted N-glycoproteins from human hepatocellular carcinoma plasma using liquid chromatography with tandem mass spectrometry

Ji E, Hwang H, Park G, Lee J, Lee H, Choi N, Jeong H, Kim K, Kim J, Lee S, Ahn Y
Analytical and Bioanalytical Chemistry (2016) vol.408 issue 27 7761-7774

Fucosylated N-glycopeptides identified in liver-secreted proteins from HCC plasma in a site-specific analysis.

Core fucose and bisecting GlcNAc, the direct modifiers of the N-glycan core: their functions and target proteins

Takahasi M, Kuroki Y, Ohtsubo K, Taniguchi N
Carbohydrate Research (2009) vol.344 issue 12 1387-1390

Artifical alteration of cloned glycosyltransferases, with a focus on Fut9 and GnT-III involved in biosynthesis of N-glycan branching, adhesion of molecules and cell surface receptors.

Bisected, complex N-glycans and galectins in mouse mammary tumor progression and human breast cancer

Miwa H, Kobe W, Fine E, Giricz O, Kenny P, Stanley P
Glycobiology (2013) vol.23 issue 12 1477-1490

Lightly touches on N-acetylglucosaminyl and effect of certain complex N-glycans in cancerous tissue in relation to bisection.

Roles of N-acetylglucosaminyltransferase III in epithelial-to-mesenchymal transition induced by transforming growth factor β1 (TGF-β1) in epithelial cell lines

Xu Q, Isaji T, Lu Y, Gu W, Kondo M, Fukada T, Du Y, Gu J
The Journal of Biological Chemistry (2012) vol.287 issue 20 16563-16574

GnT-III influenced EMT-like changes through not only prolongation of E-cadherin turnover but also suppression of β-catenin·p-Smad complex formation. GnT-III plays important roles in TGF-β-induced EMT-like changes.



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