The Helena Biosciences IgG IEF kit is intended for the identification of IgG-specific oligoclonal banding in serum and cerebro-spinal fluid (CSF) by agarose gel isoelectric focusing and immunoblotting. This technique is considered to be the “Gold Standard”1 method for the determination of intrathecal IgG synthesis in the clinical diagnosis of multiple sclerosis and offers advantages over other methods such as IgG quotient, blood-CSF barrier function, direct staining and in-gel fixation methods. Many studies have shown that direct silver staining techniques exhibit reduced sensitivity and specificity to IgG.
Having dealt with Helena for 20 years now, I am obviously convinced of their excellence. The analysis of spinal fluid has been a Cinderella area due to a combination of smaller numbers of samples yet higher levels of sophistication required to detect oligoclonal IgG patterns. Helena have maintained the highest levels of quality control. This culminated in the recognition by the US Food and Drugs Administration of their kit for spinal fluid analysis. Thus the technique was further adopted by an International group of experts on the laboratory diagnosis of Multiple Sclerosis. It remains the “gold standard” in this field. Professor E J Thompson PhD MD DSc FRCPath FRCP,
The National Hospital for Neurology & Neurosurgery
|Tests per gel:||5|
|Gels per kit:||10|
|Tests per gel:||10|
|Gels per kit:||10|
Oligoclocal banding analysis is used in combination with MRI scans to confirm Multiple Sclerosis when the situation is still clinically uncertain. The presence of oligoclonal bands in cerebro-spinal fluid (CSF) is indicative of intrathecal IgG production. This indicates an immune response to the central nervous system which is characteristic of Multiple Sclerosis.
The Helena Biosciences oligoclonal banding assay uses the Isoelectric Focusing technique (IEF). CSF-derived proteins are separated in the gel via a pH gradient which causes charged proteins to move up to the point at which their net electrical charge is neutral — known as the proteins’ Isoelectric Point (pI).
IEF in combination with immunoblotting allows the Helena Biosciences IgG method to provide clear, precise oligoclonal band detection. Our simple, step-by-step procedure provides accurate and efficient diagnosis.
Immunoblotting is one of the most sensitive methods for the detection of proteins following electrophoresis.
In addition to the gel kits, Helena Biosciences offers a control designed to ensure peak performance of all stages of the process from focusing to protein transfer to immunodetection.
|102200||SAS IgG IEF Kit (1 Step)|
|102201||SAS IgG IEF Kit (gel kit only)|
|104200||SAS IgG IEF Plus Kit (1 Step)|
|104201||SAS IgG IEF Plus Kit (gel kit only)|
Accessories required for IEF
|311105||SAS Membrane Roller|
|211400||Electrophoresis Staining Tray|
|102206||SAS Kappa IEF Kit|
|102207||SAS Lambda IEF Kit|
|102208||SAS Free Kappa IEF Kit|
|102209||SAS Free Lambda IEF Kit|
|102210||IgG IEF Control|
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