PCA Ratio

REF: 5546

Intended use

The PCA Ratio Kit is a clot based assay used in determination of resistance to activated Protein C caused by Factor V Leiden mutation. This APTT based assay creates a clotting time ratio of PCA.APTT/APTT which determines APC resistance.

Main features

  • Activates endogenous Protein C (using Protac®)
  • Excellent discrimination between APC resistant and normal samples
  • Superior result discrimination compared to traditional APC methods
  • Compatible with all instrumentation capable of carrying out APTT assays
  • Negates Lupus and Heparin interference

Kit contents

Component

REF

Volume

Stability

Factor V Depleted Plasma

5546

4 × 2 mL

Reconstituted Factor V Depleted Plasma should be discarded after use, or can be frozen at -20°C and thawed once

APTT

5546

2 × 2 mL

Reconstituted APTT Reagent is stable for 2 weeks at +2 –+6°C

PCA.APTT

5546

2 × 2 mL

Reconstituted

PCA.APTT Reagent is stable for

2 weeks at +2 –+6°C

APC Resistant Plasma

5546

1 × 0.5 mL

Reconstituted APC Resistant Plasma can be frozen and thawed

Calcium Chloride 0.025M

5546

2 × 8mL

Store at +2 –+6°C, and is stable until the expiry date indicated on the label


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